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active aurora b protein  (Millipore)

 
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    Millipore active aurora b protein
    Active Aurora B Protein, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/active aurora b protein/product/Millipore
    Average 90 stars, based on 1 article reviews
    active aurora b protein - by Bioz Stars, 2026-02
    90/100 stars

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    active aurora b protein  (Millipore)
    90
    Millipore active aurora b protein
    Active Aurora B Protein, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/active aurora b protein/product/Millipore
    Average 90 stars, based on 1 article reviews
    active aurora b protein - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    recombinant proteins kinase-active aurora b  (Millipore)
    90
    Millipore recombinant proteins kinase-active aurora b
    Phosphorylation of Ser331 is required for complete Aurora B activation. (A) Phosphorylation of Ser331 (pS331) during unperturbed prometaphase. CHO WT and CHO S331A cells expressing CENP-A–GFP were induced with tetracycline. (B) Immunoprecipitation kinase assay. CHO WT and CHO S331A cells induced with tetracycline were untreated (un) or treated with nocodazole (nocod) or taxol for 8 h in the absence or presence of VX-680. (top) Western blot analysis of Myc-associated phosphorylation of Ser10 of histone <t>H3</t> (pH3). (bottom) Western blot of immunoprecipitated (IP) Myc. (C) Densitometric analysis of pH3 levels from B. pH3 levels in sample 1 were arbitrarily set to 1. Error bars show the standard deviation from the mean from three independent experiments. The p-values were calculated using the Student’s t test. (D) Mitotic index of each sample from B at the time of harvesting (untreated or shake-off cells). (E) In vitro kinase assay. Complexes of <t>purified</t> <t>Chk1,</t> Aurora B, and GST-INCENP 826–919 were incubated with histone H3, and pH3 (top) or pS331 activities (bottom) were determined. Values show the relative levels of pH3 or pS331, and levels at 0 min were arbitrarily set to 1. Western blot analysis of total H3 and Aurora B is also shown. Black lines indicate that intervening lanes have been spliced out. (F) Coimmunoprecipitation assay. (top) Western blot analysis of immunoprecipitated (IP) 6×Myc–Aurora B (Myc) and V 5 -INCENP (V 5 ) after induction of CHO WT and CHO S331A cells with tetracycline. (bottom) Western blot analysis of total Myc, V 5 , and actin. (G) Phosphorylation of Thr232 (pT232). CHO WT and CHO S331A cells were induced with tetracycline and treated with taxol for 4 h. The frequency of cells exhibiting the respective phenotype and mean pT232/CENP-B fluorescence intensity values (boxed numbers) are shown. Insets show magnified kinetochores. WT, wild type. Bars, 5 µm.
    Recombinant Proteins Kinase Active Aurora B, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant proteins kinase-active aurora b/product/Millipore
    Average 90 stars, based on 1 article reviews
    recombinant proteins kinase-active aurora b - by Bioz Stars, 2026-02
    90/100 stars
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    Phosphorylation of Ser331 is required for complete Aurora B activation. (A) Phosphorylation of Ser331 (pS331) during unperturbed prometaphase. CHO WT and CHO S331A cells expressing CENP-A–GFP were induced with tetracycline. (B) Immunoprecipitation kinase assay. CHO WT and CHO S331A cells induced with tetracycline were untreated (un) or treated with nocodazole (nocod) or taxol for 8 h in the absence or presence of VX-680. (top) Western blot analysis of Myc-associated phosphorylation of Ser10 of histone H3 (pH3). (bottom) Western blot of immunoprecipitated (IP) Myc. (C) Densitometric analysis of pH3 levels from B. pH3 levels in sample 1 were arbitrarily set to 1. Error bars show the standard deviation from the mean from three independent experiments. The p-values were calculated using the Student’s t test. (D) Mitotic index of each sample from B at the time of harvesting (untreated or shake-off cells). (E) In vitro kinase assay. Complexes of purified Chk1, Aurora B, and GST-INCENP 826–919 were incubated with histone H3, and pH3 (top) or pS331 activities (bottom) were determined. Values show the relative levels of pH3 or pS331, and levels at 0 min were arbitrarily set to 1. Western blot analysis of total H3 and Aurora B is also shown. Black lines indicate that intervening lanes have been spliced out. (F) Coimmunoprecipitation assay. (top) Western blot analysis of immunoprecipitated (IP) 6×Myc–Aurora B (Myc) and V 5 -INCENP (V 5 ) after induction of CHO WT and CHO S331A cells with tetracycline. (bottom) Western blot analysis of total Myc, V 5 , and actin. (G) Phosphorylation of Thr232 (pT232). CHO WT and CHO S331A cells were induced with tetracycline and treated with taxol for 4 h. The frequency of cells exhibiting the respective phenotype and mean pT232/CENP-B fluorescence intensity values (boxed numbers) are shown. Insets show magnified kinetochores. WT, wild type. Bars, 5 µm.

    Journal: The Journal of Cell Biology

    Article Title: Phosphorylation at serine 331 is required for Aurora B activation

    doi: 10.1083/jcb.201104023

    Figure Lengend Snippet: Phosphorylation of Ser331 is required for complete Aurora B activation. (A) Phosphorylation of Ser331 (pS331) during unperturbed prometaphase. CHO WT and CHO S331A cells expressing CENP-A–GFP were induced with tetracycline. (B) Immunoprecipitation kinase assay. CHO WT and CHO S331A cells induced with tetracycline were untreated (un) or treated with nocodazole (nocod) or taxol for 8 h in the absence or presence of VX-680. (top) Western blot analysis of Myc-associated phosphorylation of Ser10 of histone H3 (pH3). (bottom) Western blot of immunoprecipitated (IP) Myc. (C) Densitometric analysis of pH3 levels from B. pH3 levels in sample 1 were arbitrarily set to 1. Error bars show the standard deviation from the mean from three independent experiments. The p-values were calculated using the Student’s t test. (D) Mitotic index of each sample from B at the time of harvesting (untreated or shake-off cells). (E) In vitro kinase assay. Complexes of purified Chk1, Aurora B, and GST-INCENP 826–919 were incubated with histone H3, and pH3 (top) or pS331 activities (bottom) were determined. Values show the relative levels of pH3 or pS331, and levels at 0 min were arbitrarily set to 1. Western blot analysis of total H3 and Aurora B is also shown. Black lines indicate that intervening lanes have been spliced out. (F) Coimmunoprecipitation assay. (top) Western blot analysis of immunoprecipitated (IP) 6×Myc–Aurora B (Myc) and V 5 -INCENP (V 5 ) after induction of CHO WT and CHO S331A cells with tetracycline. (bottom) Western blot analysis of total Myc, V 5 , and actin. (G) Phosphorylation of Thr232 (pT232). CHO WT and CHO S331A cells were induced with tetracycline and treated with taxol for 4 h. The frequency of cells exhibiting the respective phenotype and mean pT232/CENP-B fluorescence intensity values (boxed numbers) are shown. Insets show magnified kinetochores. WT, wild type. Bars, 5 µm.

    Article Snippet: Recombinant proteins histone H1, histone H3, Chk1, GST-INCENP 826–916 , kinase-active Aurora B, and Aurora B KD (D200A) were obtained from Millipore.

    Techniques: Phospho-proteomics, Activation Assay, Expressing, Immunoprecipitation, Kinase Assay, Western Blot, Standard Deviation, In Vitro, Purification, Incubation, Co-Immunoprecipitation Assay, Fluorescence

    Phosphorylation of Ser331 is required for TSS phosphorylation. (A) Localization of INCENP-GFP. (top) CHO WT cells expressing INCENP-GFP were induced with tetracycline. (bottom) BE cells expressing INCENP-GFP. (B and C) Phosphorylation of INCENP at Ser850 (pS850). CHO WT and CHO S331A cells expressing INCENP-GFP were induced with tetracycline and treated with taxol (B) or nocodazole (nocod, C) for 4 h. The frequency of cells exhibiting the respective phenotype and mean pS850/INCENP-GFP fluorescence intensity values (boxed numbers) are shown. (D) Immunoprecipitation kinase assay. CHO WT and CHO S331A cells expressing V 5 -INCENP were induced with tetracycline and treated with taxol for 8 h. (top) Western blot analysis of V 5 -associated phosphorylation of Ser10 of histone H3 (pH3). (middle and bottom) Western blot analysis of immunoprecipitated Myc and V 5 . Values show the relative pH3 and Myc levels with levels at sample 1 arbitrarily set to 1. (E) Densitometric analysis of pH3 levels from D. The p-values were calculated using the Student’s t test. (F) Mitotic index of each sample from D at the time of harvesting. Error bars show the standard deviation from the mean from three independent experiments. Insets show magnified kinetochores. WT, wild type. Bars, 5 µm.

    Journal: The Journal of Cell Biology

    Article Title: Phosphorylation at serine 331 is required for Aurora B activation

    doi: 10.1083/jcb.201104023

    Figure Lengend Snippet: Phosphorylation of Ser331 is required for TSS phosphorylation. (A) Localization of INCENP-GFP. (top) CHO WT cells expressing INCENP-GFP were induced with tetracycline. (bottom) BE cells expressing INCENP-GFP. (B and C) Phosphorylation of INCENP at Ser850 (pS850). CHO WT and CHO S331A cells expressing INCENP-GFP were induced with tetracycline and treated with taxol (B) or nocodazole (nocod, C) for 4 h. The frequency of cells exhibiting the respective phenotype and mean pS850/INCENP-GFP fluorescence intensity values (boxed numbers) are shown. (D) Immunoprecipitation kinase assay. CHO WT and CHO S331A cells expressing V 5 -INCENP were induced with tetracycline and treated with taxol for 8 h. (top) Western blot analysis of V 5 -associated phosphorylation of Ser10 of histone H3 (pH3). (middle and bottom) Western blot analysis of immunoprecipitated Myc and V 5 . Values show the relative pH3 and Myc levels with levels at sample 1 arbitrarily set to 1. (E) Densitometric analysis of pH3 levels from D. The p-values were calculated using the Student’s t test. (F) Mitotic index of each sample from D at the time of harvesting. Error bars show the standard deviation from the mean from three independent experiments. Insets show magnified kinetochores. WT, wild type. Bars, 5 µm.

    Article Snippet: Recombinant proteins histone H1, histone H3, Chk1, GST-INCENP 826–916 , kinase-active Aurora B, and Aurora B KD (D200A) were obtained from Millipore.

    Techniques: Phospho-proteomics, Expressing, Fluorescence, Immunoprecipitation, Kinase Assay, Western Blot, Standard Deviation